Free online tool

Sequence Alignment Viewer

Compare two short DNA sequences. Line up their bases and inspect matches, differences and gaps.

Runs on a separate serverNo account neededFASTA / AlignmentOpen workspace ↓

What does a pairwise sequence alignment show?

Pairwise alignment arranges two sequences to compare corresponding bases and gaps. This tool performs a global alignment using an explicit match, mismatch, and gap scoring scheme.

Your input

0 characters

This tool sends your input to a separate processing service. Temporary job files are deleted when processing ends. Only send content you are comfortable uploading.

Separate processing · Automatic file cleanup

Your result

A little clarity, right here.

Start with your own content or load the example. Your result will appear here.

Three simple steps

How to use Sequence Alignment Viewer

  1. Choose your input and review the processing notice.

  2. Send the job to the isolated processing service.

  3. Review the result and download your export.

Common questions

Good to know

Understand the result.
Keep the original.

Is this tool free, and are my files uploaded?

This tool is free with no account required. When you run the tool, your input goes to a separate processing service. Temporary files are deleted when the job ends. You can download the result to your device.

What are the limits and details?

Sequences are limited to 5,000 DNA bases each. The best-scoring alignment is not proof of biological function, ancestry, or a clinical finding.

Limits: one file up to 8 MB; 500,000 characters per text field. One job runs at a time on the worker, with a two-minute limit.